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Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
11:19

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Published on: February 20, 2017

Multiple domains of fission yeast Cdc19p (MCM2) are required for its association with the core MCM complex

D A Sherman1, S G Pasion, S L Forsburg

  • 1Molecular Biology and Virology Laboratory, The Salk Institute, La Jolla, California 92037, USA.

Molecular Biology of the Cell
|July 11, 1998
PubMed

Insights

Fission yeast MCM complexes, crucial for DNA replication, exhibit instability and distinct substructures. Cdc19p (MCM2) assembly into these complexes depends on Cdc21p (MCM4) and other core components.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • MCM proteins are essential eukaryotic DNA replication factors.
  • They form a six-member protein complex.
  • Understanding MCM complex structure is key to DNA replication.

Purpose of the Study:

  • Investigate the in vivo structure of fission yeast MCM complexes.
  • Determine the requirements for MCM complex formation.
  • Focus on Cdc19p (MCM2) interactions within the complex.

Main Methods:

  • Gel filtration analysis to assess complex stability.
  • Coimmunoprecipitation to study protein associations.
  • Analysis of Cdc19p mutants to identify binding domains.

Main Results:

  • MCM complexes are unstable and dissociate into subcomplexes.
  • Mis5p (MCM6) and Cdc21p (MCM4) form a stable core complex.
  • Cdc19p (MCM2) loosely associates with the core, dependent on Cdc21p.
  • Multiple Cdc19p domains are essential for MCM binding.
  • No significant cell cycle changes observed in Cdc19p-containing complexes.

Conclusions:

  • Fission yeast MCM complexes possess distinct substructures.
  • These substructures may be functionally relevant for DNA replication.
  • Cdc19p's role in MCM complex assembly is elucidated.

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