Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Osteoblastic responses to TGF-beta during bone remodeling

A Erlebacher1, E H Filvaroff, J Q Ye

  • 1Departments of Growth and Development, University of California at San Francisco, San Francisco, California 94143, USA.

Molecular Biology of the Cell
|July 11, 1998
PubMed
Summary

Transforming growth factor-beta (TGF-beta) directly promotes osteoblast differentiation, enhancing bone density. It also indirectly stimulates bone formation by influencing osteoclast activity, revealing its central role in bone remodeling.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Establishment of fetomaternal tolerance through glycan-mediated B cell suppression.

Nature·2022
Same author

Phase I study of CC-90010, a reversible, oral BET inhibitor in patients with advanced solid tumors and relapsed/refractory non-Hodgkin's lymphoma.

Annals of oncology : official journal of the European Society for Medical Oncology·2020
Same author

Karyotype analysis of Rheum palmatum.

Genetics and molecular research : GMR·2014
Same author

Literature watch. Cytotrophoblast induction of arterial apoptosis and lymphangiogenesis in an in vivo model of human placentation.

Lymphatic research and biology·2007
Same author

E7 proteins from high- and low-risk human papillomaviruses bind to TGF-beta-regulated Smad proteins and inhibit their transcriptional activity.

Archives of virology·2006
Same author

Effect of anti-TGF-beta antibodies in syngeneic mouse models of metastasis.

Journal of musculoskeletal & neuronal interactions·2005

Area of Science:

  • Cell Biology
  • Bone Biology
  • Endocrinology

Background:

  • Bone remodeling relies on coordinated osteoblast and osteoclast activity.
  • The molecular mechanisms linking bone formation and resorption are not fully understood.
  • Previous studies implicated TGF-beta2 in deregulating bone remodeling and causing bone loss.

Purpose of the Study:

  • To investigate the physiological role of TGF-beta in bone remodeling.
  • To elucidate how TGF-beta regulates osteoblast and osteoclast functions.
  • To understand the coordination of opposing bone cell activities by TGF-beta.

Main Methods:

  • Utilized transgenic mice overexpressing TGF-beta2.
  • Employed alendronate to inhibit bone resorption.
  • Used c-fos-/- mice lacking osteoclast activity.

Related Experiment Videos

  • Generated a transgenic mouse line with a dominant-negative TGF-beta type II receptor in osteoblasts.

    • Main Results:

    • TGF-beta directly increases osteoblast differentiation rate, leading to higher osteocyte density.
    • Increased bone matrix formation is a homeostatic response to TGF-beta-induced bone resorption, not a direct effect.
    • Osteoclast activity enhances TGF-beta-induced osteoblast differentiation at resorption sites.

    Conclusions:

    • TGF-beta is a key physiological regulator of osteoblast differentiation.
    • TGF-beta acts as a central mediator in coupling bone formation to resorption.
    • These findings provide insight into the molecular basis of bone remodeling and osteoporosis.