Exogenous adenosine potentiates hypnosis induced by intravenous anaesthetics

We investigated the effect of adenosine on hypnosis induced by thiopentone, propofol and midazolam in mice. The onset and duration of hypnosis were determined by the loss of righting reflex. Adenosine and 2-chloroadenosine caused a significant shortening of onset of sleep-time and prolongation of duration of sleep-time in all groups (p < 0.05). Dipyridamole administration before combined intravenous anaesthetic-adenosine or intravenous anaesthetic-2-chloroadenosine administration produced similar effects to adenosine (p < 0.05). The adenosine antagonist theophylline, given before intravenous anaesthetic-adenosine or intravenous anaesthetic-2-chloroadenosine administration caused a significant delay in onset of sleep-time and shortening in the duration of sleep-time (p < 0.05). We conclude that central excitatory noradrenergic neurones play an important role in adenosine, 2-chloroadenosine and dipyridamole-induced hypnotic responses to intravenous anaesthetics and their inhibition by adenosine antagonists.