Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interleukin-4 and interleukin-10 are chondroprotective and decrease mononuclear cell recruitment in human rheumatoid

C Jorgensen1, F Apparailly, I Couret

  • 1Service d'Immuno-Rhumatologie, Lapeyronie Hospital, Montpellier, France.

Immunology
|July 11, 1998
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Black Hole Spectroscopy and Tests of General Relativity with GW250114.

Physical review letters·2026
Same author

GW250114: Testing Hawking's Area Law and the Kerr Nature of Black Holes.

Physical review letters·2025
Same author

Predicting climate change impacts on marine fisheries, biodiversity and economy in the Canary/Iberia current upwelling system.

Journal of environmental management·2025
Same author

Validation of a new fluidic device for mechanical stimulation and characterization of microspheres: A first step towards cartilage characterization.

Materials science & engineering. C, Materials for biological applications·2021
Same author

Identification of TGFβ signatures in six murine models mimicking different osteoarthritis clinical phenotypes.

Osteoarthritis and cartilage·2020
Same author

PPARβ/δ-dependent MSC metabolism determines their immunoregulatory properties.

Scientific reports·2020

Interleukin-10 (IL-10) and Interleukin-4 (IL-4) show chondroprotective effects in rheumatoid arthritis models. IL-10 effectively reduces mononuclear cell recruitment to synovial tissue, suggesting therapeutic potential.

Area of Science:

  • Immunology
  • Rheumatology
  • Cell Biology

Background:

  • Rheumatoid arthritis (RA) is characterized by chronic inflammation and joint destruction.
  • Mononuclear cell (MNC) infiltration into the synovium drives RA pathogenesis.
  • Cartilage degradation is a hallmark of RA, leading to joint dysfunction.

Purpose of the Study:

  • To investigate the effects of IL-4 and IL-10 on cartilage degradation in vivo.
  • To assess the impact of IL-4 and IL-10 on MNC recruitment to human rheumatoid synovium.
  • To evaluate the modulation of adhesion molecule expression by IL-4 and IL-10.

Main Methods:

  • Utilized a severe combined immunodeficient (SCID) mouse model engrafted with human rheumatoid synovium and cartilage.
  • Administered recombinant human IL-4, IL-10, or TNF-alpha to the grafts.

Related Experiment Videos

  • Assessed cartilage degradation via computerized image analysis.
  • Quantified MNC recruitment using indium-111 labeled blood MNC and gamma camera imaging.
  • Analyzed intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin expression via immunochemistry.

    • Main Results:

    • IL-4, IL-10, and combined IL-4/IL-10 injections significantly inhibited cartilage degradation compared to controls.
    • IL-10 and combined IL-4/IL-10 treatments markedly reduced MNC activity in the synovial graft.
    • IL-10 significantly decreased ICAM-1 expression on synovial cells, while IL-4 did not affect ICAM-1, VCAM-1, or E-selectin.

    Conclusions:

    • IL-4 and IL-10 demonstrate chondroprotective properties in an in vivo model of rheumatoid arthritis.
    • IL-10 exhibits potent inhibition of MNC trafficking to the synovium.
    • These findings suggest IL-10 and IL-4 as potential therapeutic agents for managing rheumatoid arthritis progression.