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Updated: Aug 22, 2026

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo
Published on: January 14, 2016
Molecular interaction between COP1 and HY5 defines a regulatory switch for light control of Arabidopsis development
L H Ang1, S Chattopadhyay, N Wei
1Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA.
Abstract:
Arabidopsis COP1 acts as a light-inactivable repressor of photomorphogenic development, but its molecular mode of action remains unclear. Here, we show that COP1 negatively regulates HY5, a bZIP protein and a positive regulator of photomorphogenic development. Both in vitro and in vivo assays indicate that COP1 interacts directly and specifically with HY5. The hyperphotomorphogenic phenotype caused by the over-expression of a mutant HY5, which lacks the COP1-interactive domain, supports the regulatory role of HY5-COP1 interaction. Further, HY5 is capable of directly interacting with the CHS1 minimal promoter and is essential for its light activation. We propose that the direct interaction with and regulation of transcription factors by COP1 may represent the molecular mechanism for its control of gene expression and photomorphogenic development.
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