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[Properties of the tick-borne encephalitis virus persisting for a long time in a chronically infected cell culture]
Abstract:
The biological properties of tick-borne encephalitis virus (Sof K) produced by chronically infected HEp-2-Sof cells at late stages of the cultivation were studied. The Sof K virus was infective for mice of different ages upon intracerebral and subcutaneous inoculation, had high immunogenicity and was completely neutralized by antiserum to the parental strain. The Sof K virus had the small-plaque phenotype and replicated with similar intensity in pig embryo cell cultures (SPEC) at 36 degrees, 42 degrees and 31 degrees C.
Insights
This study investigated tick-borne encephalitis virus (TBEV) properties from infected cells. The TBEV strain showed infectivity in mice and high immunogenicity, neutralized by specific antiserum.
Area of Science:
- Virology
- Infectious Diseases
- Cell Biology
Context:
- Tick-borne encephalitis virus (TBEV) is a significant human pathogen.
- Chronic HEp-2-Sof cell cultures provide a model for studying TBEV evolution.
- Late-stage cultivation can alter viral properties.
Purpose:
- To characterize the biological properties of TBEV (Sof K) produced from chronically infected cells.
- To assess the infectivity, immunogenicity, and neutralization of the Sof K strain.
- To evaluate the replication capacity of Sof K in different temperature conditions.
Summary:
- The Sof K TBEV strain, derived from late-stage infected HEp-2-Sof cells, demonstrated infectivity in mice via intracerebral and subcutaneous routes.
- This strain exhibited high immunogenicity and was fully neutralized by antiserum against the parent strain.
- Sof K displayed a small-plaque phenotype and consistent replication across 31°C, 36°C, and 42°C in pig embryo cell cultures (SPEC).
Impact:
- Provides insights into the biological characteristics of TBEV variants from chronic infections.
- Contributes to understanding TBEV adaptation and potential changes in virulence.
- Informs future research on TBEV pathogenesis and vaccine development.