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FISH: recent advances and diagnostic aspects
1Department of Pathology, Maimonides Medical Center, Brooklyn, USA.
Cell Vision : the Journal of Analytical Morphology
|July 11, 1998
Summary
Fluorescence in situ Hybridization (FISH) visualizes nucleic acid sequences, aiding in disease diagnostics and monitoring. Recent modifications expand its applications in research and medicine.
Area of Science:
- Molecular Biology
- Genetics
- Cytology
Background:
- In situ Hybridization (ISH) visualizes nucleic acid sequences without altering cellular integrity.
- Fluorescence in situ Hybridization (FISH) uses fluorescent probes for bright signal detection.
- FISH is crucial for identifying genetic aberrations linked to disease.
Purpose of the Study:
- To review Fluorescence in situ Hybridization (FISH) techniques.
- To discuss modifications and practical applications of FISH.
- To evaluate innovative FISH-based methods.
Main Methods:
- Review of existing literature on FISH and its modifications.
- Discussion of techniques such as Chromosome Painting, Multicolor FISH, and CGH.
- Exploration of applications in research, diagnostics, and disease monitoring.
Main Results:
- FISH enables precise localization of genetic and genomic aberrations.
- Numerous FISH modifications enhance its utility in various fields.
- Techniques like FICTION and PRINS offer advanced investigative capabilities.
Conclusions:
- FISH is an indispensable tool in molecular diagnostics and research.
- Continuous innovation in FISH technology broadens its practical applications.
- FISH modifications are vital for understanding disease mechanisms and progression.