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Related Experiment Videos

CDC16 controls initiation at chromosome replication origins

K A Heichman1, J M Roberts

  • 1Howard Hughes Medical Institute, Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.

Molecular Cell
|July 14, 1998
PubMed
Summary
This summary is machine-generated.

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The CDC16 gene is crucial for preventing DNA rereplication in budding yeast. Mutations in CDC16 allow DNA synthesis to restart inappropriately, even when cell cycle progression is blocked.

Area of Science:

  • Cell biology
  • Molecular genetics
  • Yeast genetics

Background:

  • Cdc28p cyclin-dependent kinase regulates DNA replication initiation and prevents rereplication.
  • CDC16 gene mutations in budding yeast lead to DNA rereplication despite G2 arrest and high Cdc28p activity.

Purpose of the Study:

  • To investigate the nature of DNA accumulation in cdc16 mutants.
  • To determine the mechanism by which CDC16 prevents DNA rereplication.

Main Methods:

  • Two-dimensional DNA electrophoresis to analyze DNA replication origins.
  • Density transfer experiments to track DNA synthesis.
  • Analysis of initiation proteins Cdc6p and Cdc46/Mcm5p.

Main Results:

Related Experiment Videos

  • Accumulated DNA in cdc16 mutants is primarily chromosomal.
  • cdc16 mutants reinitiate DNA synthesis from normal replication origins.
  • Rereplication requires initiation proteins Cdc6p and Cdc46/Mcm5p, which are normally inactivated by Cdc28p.

    • Conclusions:

    • CDC16 is essential for preventing the inappropriate firing of DNA replication origins.
    • The findings challenge the established model of Cdc28p-mediated control of DNA replication.
    • CDC16 acts as a critical regulator to ensure proper cell cycle progression and prevent DNA rereplication.