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Related Experiment Videos

Unrestraining genetic processes with a protein-DNA hinge

T Tomonaga1, G A Michelotti, D Libutti

  • 1Laboratory of Pathology, National Cancer Institute, Bethesda, Maryland 20892, USA.

Molecular Cell
|July 14, 1998
PubMed
Summary

DNA flexibility is crucial for gene regulation, enabling protein interactions at distant sites. Researchers found a protein-DNA hinge in the human c-myc gene that facilitates this process.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Gene regulation relies on protein interactions with DNA.
  • The rigidity of DNA poses challenges for bringing distant DNA-binding proteins together.

Purpose of the Study:

  • To investigate mechanisms that facilitate protein-protein interactions at nonadjacent DNA sites.
  • To identify DNA structures that promote the juxtaposition of separated cis elements.

Main Methods:

  • Examination of a specific cis element in the human c-myc gene.
  • Analysis of the associated single-strand DNA binding protein.
  • Investigating DNA bending and twisting properties.

Main Results:

  • A protein-DNA hinge was identified within the human c-myc gene.

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  • This hinge structure, involving a melted DNA region and a binding protein, acts as a universal joint.
  • It facilitates the association of separated DNA-binding proteins regardless of their position.

    • Conclusions:

    • The identified protein-DNA hinge provides a general mechanism for overcoming DNA rigidity.
    • This structure is essential for enabling protein interactions required for genetic processes.
    • The findings offer insights into DNA flexibility and its role in gene regulation.