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Updated: Jul 24, 2026

Dechorionation of Medaka Embryos and Cell Transplantation for the Generation of Chimeras
Published on: December 22, 2010
Transgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells
J B Cibelli1, S L Stice, P J Golueke
1Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst 01003, USA.
Abstract:
We have developed a method, using nuclear transplantation, to produce transgenic embryonic stem (ES)-like cells from fetal bovine fibroblasts. These cells, when reintroduced into preimplantation embryos, differentiated into derivatives from the three embryonic germ layers, ectoderm, mesoderm, and endoderm, in 5-month-old animals. Six out of seven (86%) calves born were found to be chimeric for at least one tissue. These experiments demonstrate that somatic cells can be genetically modified and then de-differentiated by nuclear transfer into ES-like cells, opening the possibility of using them in differentiation studies and human cell therapy.
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