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Related Experiment Videos

Iodide symporter gene expression in human thyroid tumors

F Arturi1, D Russo, M Schlumberger

  • 1Dipartimento di Medicina Sperimentale e Clinica, Facoltà di Farmacia, Università di Catanzaro, Italy.

The Journal of Clinical Endocrinology and Metabolism
|July 14, 1998
PubMed
Summary

Loss of Na+/I- symporter (NIS) gene expression in primary thyroid cancer may indicate poor response to radioactive iodine therapy. Early detection of this NIS gene defect could aid in managing differentiated thyroid cancer.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Endocrinology

Background:

  • The Na+/I- symporter (NIS) is crucial for thyroid hormone synthesis and radioiodine uptake.
  • Understanding NIS gene expression in thyroid tumors is vital for predicting treatment response.

Purpose of the Study:

  • To investigate Na+/I- symporter (NIS) gene expression in various thyroid tumors.
  • To correlate NIS expression with tumor type and metastatic potential.
  • To assess the diagnostic and prognostic value of NIS gene expression in differentiated thyroid cancer.

Main Methods:

  • Reverse transcription-polymerase chain reaction (RT-PCR) was used to analyze NIS messenger ribonucleic acid (mRNA) expression.
  • Dot blot analysis quantified NIS mRNA levels in primary tumors and metastases.

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  • Thyroid carcinomas and adenomas were analyzed, including papillary, follicular, and anaplastic types.

    • Main Results:

    • NIS mRNA was expressed in most follicular adenomas and follicular thyroid cancers.
    • NIS expression was reduced or absent in anaplastic thyroid cancers and some papillary thyroid cancers.
    • Reduced NIS mRNA expression in primary tumors correlated with metastases and negative post-therapy 131I scans.
    • Loss of NIS expression in metastases was an intrinsic defect of primary cancer cells, not dedifferentiation.

    Conclusions:

    • Loss of NIS gene expression is a significant finding in differentiated thyroid cancer, particularly in cases with metastases.
    • This defect is an intrinsic molecular alteration of the primary tumor.
    • Early detection of absent NIS gene expression in primary thyroid cancer may improve patient management and treatment strategies.