Related Experiment Video
Updated: Aug 18, 2026

Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae
Published on: April 18, 2016
[Threshold mechanism of the control of cascade proteolysis]
1Pavlov State Medical University, St. Petersburg, Department of Biochemistry, Russia. igshc@spmu.rssi.ru
Abstract:
The immune lysis rate of erythrocytes vs. the concentration of particular complement components was studied. It was found that in the presence of component C1 (but not C3) and factors B and D, the cells undergo lysis after the concentration of these compounds exceeds a "threshold" value. The threshold molar concentrations of factors D and B exceeded 5- and 20-fold, respectively, that of component C1. The multiplicity and different capacities of the thresholds allow the fragmentary activation of cascade proteolysis at stretches between neighboring thresholds without the total realization of the final effect of the system (cell lysis, clot formation, etc.). The resulting peptideby-products (bypass peptides) may possess their own biological activity. It is the generation of various bioregulators that appears to be the main function of the cascade proteolytic systems functioning in the subthreshold regime.
Related Concept Videos
The Proteasome
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
Regulated Protein Degradation
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Caspases
Amplifying Signals via Enzymatic Cascade
MAPK Signaling Cascades
The Proteasome
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3 (ubiquitin...

