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Updated: May 22, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
Published on: August 21, 2014
Characterization of a mammalian cDNA encoding a protein with high sequence similarity to the Drosophila regulatory
1The Babraham Institute, Cambridge, UK. john.pascall@bbsrc.ac.uk
Abstract:
The Drosophila regulatory protein Rhomboid has been demonstrated genetically to facilitate signalling within the Spitz/epidermal growth factor receptor/mitogen-activated protein kinase pathway. Using a polymerase chain reaction (PCR)-based strategy, we have cloned a human cDNA which encodes a protein that has high sequence similarity to Rhomboid. The encoded protein, termed rhomboid-related protein (RRP), is predicted to contain seven transmembrane domains. Northern analysis indicates that RRP mRNA is expressed at highest levels in brain and kidney.
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