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Updated: Jul 23, 2026

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
Published on: October 1, 2009
[Lectin-reactive immune complexes in plasma in ENT diseases]
Immune complexes (IC) reactive to PHA and WGA lectins were elevated in patients with laryngeal carcinoma and scleroma, respectively. ConA-reactive IC levels remained consistent across all groups, indicating specific lectin interactions in ENT diseases.
Area of Science:
- Immunology
- Biochemistry
- Otolaryngology
Background:
- Circulating immune complexes (IC) play a role in various pathological conditions.
- Plant lectins like Canavalia ensiformis (Con A), Phaseolus vulgaris (PHA), and Triticum vulgaris (WGA) can bind to specific carbohydrate structures on IC.
- Understanding lectin-reactive IC profiles may offer insights into disease mechanisms.
Purpose of the Study:
- To investigate the levels of PHA-, WGA-, and ConA-reactive IC in patients with head and neck diseases.
- To compare these levels with those in healthy individuals.
Main Methods:
- Blood plasma samples were collected from healthy donors and patients with ENT diseases (n=36).
- The content of circulating immune complexes interacting with Con A, PHA, and WGA was quantified.
Main Results:
- Patients with laryngeal carcinoma showed significantly higher levels of PHA- and WGA-reactive IC compared to controls.
- Patients with scleroma exhibited elevated levels of WGA-reactive IC only.
- Levels of ConA-reactive IC were statistically similar between healthy individuals and patients with either laryngeal carcinoma or scleroma.
Conclusions:
- Specific lectin-reactive immune complexes, particularly PHA- and WGA-reactive IC, may serve as biomarkers for laryngeal carcinoma and scleroma.
- ConA-reactive IC do not appear to be significantly altered in these ENT conditions.
- Further research is warranted to explore the diagnostic and prognostic potential of lectin-binding IC in otolaryngology.
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