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Intracytoplasmic sperm injection experiments using the mouse as a model

R Yanagimachi1

  • 1Department of Anatomy and Reproductive Biology, University of Hawaii Medical School, Honolulu 96822, USA.

Human Reproduction (Oxford, England)
|July 15, 1998
PubMed
Summary
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Human reproduction (Oxford, England)·2012

Mice are excellent models for intracytoplasmic sperm injection (ICSI), enabling fertilization with various sperm types. ICSI overcomes issues with sperm morphology and motility, suggesting genomic integrity is often maintained despite these defects.

Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Genetics

Background:

  • The mouse is a well-established model organism for reproductive studies due to extensive background data.
  • Intracytoplasmic sperm injection (ICSI) is a key assisted reproductive technology.

Purpose of the Study:

  • To evaluate the mouse as a model for ICSI.
  • To investigate the developmental potential of various male germ cell types via ICSI.
  • To explore the role of sperm-borne factors in oocyte activation and the impact of sperm morphology/motility on fertilization.

Main Methods:

  • Intracytoplasmic sperm injection (ICSI) was performed using mature and immature spermatozoa, round spermatids, and secondary spermatocytes from mice.
  • Spermatozoa with abnormal head morphology and poor motility were utilized for ICSI.

Related Experiment Videos

  • Oocyte activation and subsequent embryonic development were assessed.
  • Human sperm chromosomes were examined after injection into mouse oocytes.

    • Main Results:

    • Normal fertile mouse offspring were produced using ICSI with diverse germ cell types, indicating complete genomic imprinting before spermiogenesis.
    • A sperm-borne oocyte-activating factor appears during spermiogenesis, as it's present in testicular sperm but not round spermatids.
    • ICSI enabled fertilization and development of offspring from spermatozoa with misshapen heads or poor motility, which are otherwise infertile.
    • Spermatozoa with abnormal morphology showed a higher incidence of chromosomal abnormalities, but most had normal chromosomal constitutions.

    Conclusions:

    • The mouse is a suitable model for ICSI research.
    • ICSI can overcome fertilization barriers related to sperm morphology and motility.
    • Aberrant sperm morphology or motility does not necessarily indicate underlying genomic abnormalities.