Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple calvarial defects in lmx1b mutant mice

H Chen1, D Ovchinnikov, C L Pressman

  • 1Department of Biochemistry and Molecular Biology, UT MD Anderson Cancer Center, Houston 77030, USA.

Developmental Genetics
|July 17, 1998
PubMed
Summary

The LIM-homeodomain gene, lmx1b, is crucial for skull bone development and suture formation in mice. Its absence leads to missing or malformed cranial bones and abnormal sutures, impacting skull growth.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Standardizing a method for functional assessment of neural networks in brain organoids.

Journal of neuroscience methods·2024
Same author

Correction to: A PRISMA-compliant systematic review of the endpoints employed to evaluate symptomatic treatments for primary headaches.

The journal of headache and pain·2019
Same author

A PRISMA-compliant systematic review of the endpoints employed to evaluate symptomatic treatments for primary headaches.

The journal of headache and pain·2018
Same author

LMX1B, a LIM homeodomain class transcription factor, is necessary for normal development of multiple tissues in the anterior segment of the murine eye.

Genesis (New York, N.Y. : 2000)·2000
Same author

Dynamic expression of lunatic fringe suggests a link between notch signaling and an autonomous cellular oscillator driving somite segmentation.

Developmental biology·1999
Same author

lunatic fringe is an essential mediator of somite segmentation and patterning.

Nature·1998

Area of Science:

  • Developmental Biology
  • Molecular Genetics
  • Craniofacial Development

Background:

  • Calvarial vault forms from cranial mesenchyme with contributions from multiple embryonic origins.
  • Cranial sutures form via local inhibition of osteogenic potential, enabling postnatal skull growth.
  • Molecular mechanisms of calvarial bone patterning and suture specification remain largely unknown.

Purpose of the Study:

  • Investigate the function and expression of the LIM-homeodomain gene, lmx1b, in calvarial development.
  • Determine lmx1b's role in the patterning and morphogenesis of the cranial vault and sutures.

Main Methods:

  • Analysis of lmx1b expression in neuroepithelium and cranial mesenchyme during skull development.
  • Phenotypic analysis of lmx1b mutant mice to assess calvarial bone formation and suture integrity.

Related Experiment Videos

Main Results:

  • Lmx1b is expressed in the neuroepithelium and cranial mesenchyme relevant to calvarial development.
  • lmx1b is essential for the formation of supraoccipital and interparietal bones, which are reduced or absent in mutants.
  • lmx1b deficiency results in severely abnormal cranial sutures between frontal, parietal, and interparietal bones.

Conclusions:

  • Lmx1b plays a critical role in multiple aspects of vertebrate calvarial development, including bone patterning and suture formation.
  • These findings highlight lmx1b as a key regulator of skull morphogenesis.
  • Further research may explore genetic interactions between lmx1b and other skull development regulators.