Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Eotaxin and capping protein in experimental vasculopathy

J Chen1, L M Akyürek, B Fellström

  • 1Department of Medicine, St. Michael's Hospital and University of Toronto, Ontario, Canada.

The American Journal of Pathology
|July 17, 1998
PubMed
Summary

Prolonged cold storage of vascular grafts increases capping protein and eotaxin mRNA. These molecules may play a role in graft vasculopathy, offering potential therapeutic targets.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Attenuation of Torque teno viral load over time in kidney transplantation recipients treated with calcineurin inhibitors is mitigated after conversion to belatacept.

Journal of medical virology·2024
Same author

NT-proBNP and stem cell factor plasma concentrations are independently associated with cardiovascular outcomes in end-stage renal disease hemodialysis patients.

European heart journal open·2023
Same author

Adherence of Renal Transplant Recipients to Once-daily, Prolonged-Release and Twice-daily, Immediate-release Tacrolimus-based Regimens in a Real-life Setting in Sweden.

Transplantation proceedings·2020
Same author

Adherence of Renal Transplant Recipients to Once-daily, Prolonged-Release and Twice-daily, Immediate-release Tacrolimus-based Regimens in a Real-life Setting in Sweden.

Transplantation proceedings·2018
Same author

Single Nucleotide Polymorphisms and Long-Term Clinical Outcome in Renal Transplant Patients: A Validation Study.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2016
Same author

Immunological profiling of haemodialysis patients and young healthy individuals with implications for clinical regulatory T cell sorting.

Scandinavian journal of immunology·2015

Area of Science:

  • Vascular Biology
  • Transplantation Immunology
  • Molecular Medicine

Background:

  • Ischemia-induced tissue activation contributes to graft vasculopathy.
  • Mediators of this process are not fully understood.
  • Cold storage is a critical factor in graft survival.

Purpose of the Study:

  • To identify molecular mechanisms of cold-storage-induced vasculopathy.
  • To investigate differentially expressed genes in ischemic vascular grafts.
  • To explore the role of capping protein and eotaxin in graft remodeling.

Main Methods:

  • Syngeneic rat aortic transplants subjected to 1 or 18 hours of cold ischemia.
  • Differential display of mRNA to identify gene expression changes.
  • Cloning, Northern blot, and immunohistochemistry to validate findings.

Related Experiment Videos

  • Comparison with balloon denudation injury models.

    • Main Results:

    • 18 hours of ischemia significantly altered mRNA expression compared to 1 hour.
    • Capping protein and eotaxin mRNA levels were upregulated after prolonged ischemia.
    • Protein expression of capping protein-alpha and eotaxin increased, but less than mRNA.
    • Upregulation was specific to ischemic injury, not general injury response.

    Conclusions:

    • Capping protein and eotaxin are upregulated in vascular grafts by prolonged cold ischemia.
    • These molecules may contribute to ischemia-induced vessel wall remodeling.
    • Further research is needed to determine their therapeutic potential in graft vasculopathy.