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Related Experiment Videos

Loss of cell viability dramatically elevates cell surface plasminogen binding and activation

M J O'Mullane1, M S Baker

  • 1Department of Biological Sciences, University of Wollongong, NSW, Australia.

Experimental Cell Research
|July 17, 1998
PubMed
Summary

Apoptotic and dead cells bind significantly more plasminogen, but plasminogen activation primarily occurs on apoptotic cells due to increased urokinase plasminogen activator. This highlights the plasminogen activation cascade

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Area of Science:

  • Cellular biology
  • Biochemistry
  • Hematology

Background:

  • The plasminogen activation cascade is crucial for various physiological processes.
  • Cell surface receptors concentrate plasminogen and its activators.
  • U937 cells are a relevant model for studying these interactions.

Purpose of the Study:

  • To investigate plasminogen binding and activation on different U937 cell subpopulations (viable, apoptotic, dead).
  • To determine the relationship between cell viability and plasminogen interactions.
  • To elucidate the role of urokinase plasminogen activator in these processes.

Main Methods:

  • Flow cytometry was used to quantify plasminogen binding.
  • Propidium iodide uptake assessed cell viability.

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  • Plasminogen activation assays were performed on cell surface.

    • Main Results:

    • Apoptotic and dead cells exhibited up to 100-fold higher lysine-dependent plasminogen binding than viable cells.
    • Plasminogen activation was minimal on dead cells despite high binding, due to low urokinase plasminogen activator (uPA).
    • Apoptotic cells showed significant plasminogen activation, correlating with increased lysine-dependent binding and endogenous uPA.

    Conclusions:

    • Colocalization of plasminogen and uPA is essential for cell-surface plasminogen activation.
    • The plasminogen activation cascade is implicated in apoptosis, particularly in uPA-expressing cells.
    • Flow cytometry is a valuable tool for studying cell-surface plasminogen dynamics.