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Molecular cloning of a chicken JAK homolog from activated T cells

L Sofer1, D Kampa, J Burnside

  • 1University of Delaware, Department of Animal, Food Sciences, Department of Chemistry, Biochemistry, Newark, DE 19717, USA.

Gene
|July 17, 1998
PubMed

Insights

Researchers identified a chicken JAK-related protein (cJAK), an avian homolog of JAK3, predominantly expressed in immune organs and activated by T-cell stimulation.

Area of Science:

  • Immunology
  • Molecular Biology
  • Biochemistry

Background:

  • The Janus kinase (JAK) family is crucial for cytokine signaling in immune responses.
  • Understanding avian JAKs provides insights into the evolution of immune signaling pathways.

Purpose of the Study:

  • To isolate and characterize a chicken JAK-related protein.
  • To determine its relationship to mammalian JAKs and its expression pattern.

Main Methods:

  • Screening a chicken T-cell cDNA library using PCR-based probes.
  • Sequence analysis of the isolated cDNA and encoded protein.
  • Analyzing tissue-specific and T-cell activation-dependent gene expression.

Main Results:

  • Isolation of a cDNA encoding a 123 kDa protein, designated chicken JAK (cJAK).
  • cJAK possesses a double catalytic domain characteristic of JAK tyrosine kinases.
  • Sequence identity with mammalian JAK3 (59%) and JAK2 (52%) was observed.
  • cJAK is primarily expressed in the thymus and spleen, with increased levels upon T-cell activation.

Conclusions:

  • cJAK represents an avian homolog of JAK3.
  • Its expression pattern suggests a role in avian immune responses, particularly in T-cells.

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