Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Total structures of colistin minor components

Y Ikai1, H Oka, J Hayakawa

  • 1Aichi Prefectural Institute of Public Health, Nagoya, Japan.

The Journal of Antibiotics
|July 17, 1998
PubMed
Summary

Structural analysis revealed four minor components of colistin (CL). These compounds differ from main colistin A or B by substituting L-leucine with L-valine or L-isoleucine.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Noninvasive endoscopical measurement of gastric mucosal blood flow in the anesthetized rat.

Digestion·1996
Same author

Long-term corticosteroid therapy for gastric antral vascular ectasia.

The American journal of gastroenterology·1996
Same author

Equivalent parental distribution of frequently lost alleles and biallelic expression of the H19 gene in human testicular germ cell tumors.

Japanese journal of cancer research : Gann·1996
Same author

[Percutaneous microwave coagulation therapy for hepatocellular carcinoma--study on its therapeutic effect in surgical cases].

Nihon Shokakibyo Gakkai zasshi = The Japanese journal of gastro-enterology·1996
Same author

E-cadherin mediated adhesion system in cancer cells.

Cancer·1996
Same author

[A case of the hypoplasia of the hepatic left lobe complicated with cholangiocarcinoma].

Nihon Shokakibyo Gakkai zasshi = The Japanese journal of gastro-enterology·1996

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Pharmacology

Background:

  • Colistin (CL) is a crucial antibiotic for treating multidrug-resistant Gram-negative bacterial infections.
  • Understanding the structural heterogeneity of colistin is essential for optimizing its therapeutic use and developing new analogs.
  • Previous studies have primarily focused on the main components of colistin.

Purpose of the Study:

  • To elucidate the detailed structures of minor components within the colistin complex.
  • To determine the absolute configuration of amino acids in these minor components.
  • To compare the structures of minor components with the main colistin A and B.

Main Methods:

  • Frit-fast atom bombardment liquid chromatography/mass spectrometry (Frit-FAB LC/MS) for component separation and mass analysis.

Related Experiment Videos

  • Tandem mass spectrometry (MS/MS) for structural fragmentation and sequencing.
  • Marfey's amino acid analysis for determining the absolute configuration of constituent amino acids.

    • Main Results:

    • The study successfully characterized four minor colistin components.
    • The absolute configurations of the amino acids within these minor components were determined.
    • Minor components were structurally similar to colistin A or B, with L-leucine replaced by L-valine or L-isoleucine.

    Conclusions:

    • The structural elucidation of minor colistin components provides a deeper understanding of colistin's composition.
    • Identification of these variants may have implications for colistin's efficacy and resistance mechanisms.
    • This detailed structural information can guide the development of novel colistin-based antibiotics.