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Two-dimensional chromatography in the analysis of complex glycans from transferrin
J Charlwood1, H Birrell, D Tolson
1SmithKline Beecham Pharmaceuticals, New Frontiers Science Park, Harlow, Essex, UK.
Analytical Chemistry
|July 17, 1998
Abstract:
Oligosaccharides released from human transferrin have been derivatized with 2-aminoacridone (2-AMAC) prior to analysis by either reversed- or normal-phase high-performance liquid chromatography. These separation methods are complementary and allow distinction between isomeric mono- and disialylated oligosaccharides, which terminate with Sia alpha 2-6Gal or Sia alpha 2-3Gal at the nonreducing end. Collected fractions of 2-AMAC-derivatized glycans were analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, before and after desialylation.