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Insulin increases expression of apobec-1, the catalytic subunit of the apolipoprotein B mRNA editing complex in rat
M A von Wronski1, K I Hirano, L M Cagen
1Department of Pharmacology, The Health Science Center, University of Tennessee, Memphis 38163, USA.
Abstract:
We have previously shown that chronic insulin treatment of rat hepatocytes increases the fraction of edited apolipoprotein B (apoB) mRNA from approximately 50% to as much as 90%. We have now examined the effect of insulin on apobec-1 mRNA abundance and demonstrate that increased editing of apoB mRNA following insulin treatment is accompanied by elevated apobec-1 mRNA levels in primary rat hepatocytes. Time-course measurements of the effects of insulin on apoB mRNA editing and apobec-1 mRNA abundance showed that both were elevated almost maximally within 48 hours and sustained for at least 5 days of insulin treatment.
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