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Kinetoplastid RNA editing: complexes and catalysts

K Stuart¹, T E Allen, M L Kable

¹Seattle Biomedical Research Institute, 4 Nickerson Street, WA 98109-1651, USA. kstuart@u.washington.edu.

Current Opinion in Chemical Biology

|July 17, 1998

Summary

RNA editing in kinetoplastids inserts uridylates into mitochondrial mRNAs guided by small RNAs. This complex process involves mRNA cleavage, U addition/removal, and ligation, catalyzed by a macromolecular complex.

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Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Kinetoplastids utilize RNA editing for mitochondrial mRNA maturation.
This process involves uridylate (U) insertion/deletion guided by small RNAs.
The precise biochemical mechanism and enzymatic machinery are under investigation.

Purpose of the Study:

To elucidate the mechanism of RNA editing in kinetoplastids.
To characterize the macromolecular complex responsible for RNA editing.
To understand the pathway of uridylate addition/removal and ligation.

Main Methods:

In vitro studies of RNA editing.
Biochemical assays to analyze reaction intermediates.
Characterization of the macromolecular complex involved in editing.

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Main Results:

The reaction pathway involves endonucleolytic mRNA cleavage.
Uridylate addition or removal occurs at the 3' end of the 5' cleavage product.
Ligation of cleavage products completes the editing process.
RNA editing is catalyzed by an associated macromolecular complex.

Conclusions:

The general mechanism of RNA editing in kinetoplastids has been resolved.
Editing occurs within a complex macromolecular machinery.
Further characterization of this complex is needed to fully understand the process.