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Biomolecular interaction analysis: affinity biosensor technologies for functional analysis of proteins

M Malmqvist¹, R Karlsson

¹Biacore AB Rapsgatan 7, S-754 50, Uppsala, Sweden. Magnus.Malmqvist@eu.biacore.com

Current Opinion in Chemical Biology

|July 17, 1998

Summary

Label-free biosensors enable real-time analysis of biomolecular interactions. Technologies like BIACORE and IAsys are key for determining binding kinetics and affinity constants.

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Area of Science:

Biochemistry
Biophysics
Analytical Chemistry

Background:

Affinity-based biosensors offer label-free, real-time analysis of biomolecular interactions.
Established technologies like BIACORE and IAsys are widely employed in this field.

Purpose of the Study:

To highlight the capabilities of affinity-based biosensors for functional analysis.
To emphasize their application in determining kinetic and affinity constants of molecular binding.

Main Methods:

Utilizing BIACORE technology for surface plasmon resonance-based detection.
Employing IAsys technology for real-time biomolecular interaction analysis.

Main Results:

Demonstrated the effectiveness of label-free biosensing for kinetic analysis.

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Successfully determined affinity constants for various biomolecular interactions.

Conclusions:

Affinity-based biosensors provide a powerful tool for label-free, real-time characterization of biomolecular interactions.
These methods are crucial for accurately quantifying binding kinetics and affinity.