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Related Experiment Videos

High-throughput screening: advances in assay technologies

G S Sittampalam1, S D Kahl, W P Janzen

  • 1Research Technologies and Proteins, Lilly Research Laboratories, Eli Lilly and Company Indianapolis, Indiana, 46285, USA. sitta@lilly.com

Current Opinion in Chemical Biology
|July 17, 1998
PubMed
Summary

High-throughput screening (HTS) uses isotopic and nonisotopic assays for drug discovery. Advances focus on automated, homogenous assay systems and cell-based screens to identify drug candidates efficiently.

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Area of Science:

  • Biochemistry
  • Pharmacology
  • Drug Discovery

Background:

  • High-throughput screening (HTS) is crucial for identifying novel drug candidates.
  • Assay methodologies, including isotopic and nonisotopic approaches, are fundamental to HTS.
  • Recent technological advancements are enhancing the efficiency and scope of HTS.

Purpose of the Study:

  • To review recent advances in cell-based and in vitro biochemical assays for drug discovery.
  • To highlight detection technologies suitable for automated 'mix and read' procedures in HTS.
  • To discuss emerging trends in HTS assay development.

Main Methods:

  • Review of recent literature on assay methodologies in HTS.
  • Emphasis on homogenous assay systems utilizing fluorescence resonance energy transfer (FRET), fluorescence polarization (FP), and fluorescence correlation spectroscopy (FCS).

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  • Discussion of cell-based assay systems and their application in identifying disease-target modulating compounds.

    • Main Results:

    • Homogenous assay systems employing FRET, FP, and FCS represent a major trend in HTS.
    • Cell-based assays are increasingly utilized to identify compounds that directly modulate disease targets.
    • Colorimetric and amperometric methods show potential but are not yet widely adopted in HTS.

    Conclusions:

    • Advancements in assay technologies are significantly improving drug discovery through HTS.
    • Homogenous and cell-based assay systems offer powerful tools for efficient identification of active compounds.
    • Continued development of detection technologies will further optimize HTS processes.