Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cytochrome P450 monooxygenases

L L Wong1

  • 1Department of Chemistry, Inorganic Chemistry Laboratory, South Parks Road, Oxford, OX1 3QR, UK. luet.wong@chem.ox.ac.uk

Current Opinion in Chemical Biology
|July 17, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Urgent Liver Transplantation for Dietary Supplements: An Under-Recognized Problem.

Transplantation proceedings·2017
Same author

Sodium-ion diffusion mechanisms in the low cost high voltage cathode material Na(2+δ)Fe(2-δ/2)(SO4)3.

Physical chemistry chemical physics : PCCP·2015
Same author

A validation study of the Hong Kong version of Montreal Cognitive Assessment (HK-MoCA) in Chinese older adults in Hong Kong.

Hong Kong medical journal = Xianggang yi xue za zhi·2014
Same author

Pancreatic resections in renal failure patients: is it worth the risk?

HPB surgery : a world journal of hepatic, pancreatic and biliary surgery·2014
Same author

Fracture-induced subbandgap absorption as a precursor to optical damage on fused silica surfaces.

Optics letters·2010
Same author

Structural information from orientationally selective DEER spectroscopy.

Physical chemistry chemical physics : PCCP·2009

Researchers are advancing P450 enzyme studies by re-engineering bacterial enzymes for altered specificity and expressing human P450s. This progress enhances understanding and opens new research avenues in P450 chemistry and molecular biology.

Area of Science:

  • Biochemistry and Molecular Biology
  • Enzymology
  • Protein Engineering

Background:

  • Cytochrome P450 (P450) enzymes are crucial in metabolism and drug development.
  • Understanding P450s is key to fields ranging from medicine to environmental science.
  • Rapid advancements in P450 research necessitate updated summaries of progress.

Purpose of the Study:

  • To highlight recent progress in P450 enzyme research.
  • To discuss the re-engineering of bacterial P450 active sites.
  • To cover the functional expression and application of human P450 enzymes.

Main Methods:

  • Rational re-engineering of bacterial P450 active sites.
  • Alteration of substrate specificity and oxidation selectivity.
  • Functional expression of human P450 enzymes in various systems.

Related Experiment Videos

Main Results:

  • Modified bacterial P450s exhibit altered substrate specificity and selectivity.
  • Successful functional expression of numerous human P450 enzymes.
  • Development of novel methods for assessing P450 substrate turnover.

Conclusions:

  • Increased understanding of P450 chemistry and molecular biology drives innovation.
  • Re-engineered P450s offer new tools for biochemical research.
  • Advances in human P450 expression expand possibilities for drug metabolism studies.