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A method for the 32P labeling of peptides or peptide nucleic acid oligomers
I A Kozlov1, P E Nielsen, L E Orgel
1The Salk Institute for Biological Studies, P.O. Box 85800, San Diego, California 92186, USA.
Bioconjugate Chemistry
|July 21, 1998
Abstract:
A novel approach to the radioactive labeling of peptides and PNA oligomers is described. It is based on the conjugation of a deoxynucleoside 3'-phosphate with the terminal amine of the substrate, followed by phosphorylation of the 5'-hydroxyl group of the nucleotide using T4 polynucleotide kinase and [gamma-32P]ATP.