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Toxin production by Pasteurella granulomatis

H P Veit1, D J Wise, G R Carter

  • 1Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg 24061-0442, USA.

Annals of the New York Academy of Sciences
|July 21, 1998
PubMed
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Pasteurella granulomatis, a bacterium causing lechiguana in cattle, exhibits variable hemolytic and leukotoxic activities. These properties may influence the severity of the disease in affected animals.

Area of Science:

  • Veterinary Microbiology
  • Bacterial Pathogenesis

Background:

  • Pasteurella granulomatis (Pg) is a newly identified bacterium linked to proliferative fibrogranulomatous panniculitis (lechiguana) in Brazilian cattle.
  • Previous attempts to replicate lechiguana experimentally have yielded limited success, suggesting other virulence factors may be involved.

Purpose of the Study:

  • To investigate the hemolytic and leukotoxic potential of selected Pasteurella granulomatis isolates.
  • To assess the contribution of these toxins to the pathogenicity of Pg in cattle.

Main Methods:

  • Seven Pg isolates were tested for hemolytic activity on ovine and bovine blood agar plates.
  • The CAMP test was employed to confirm hemolytic activity on bovine blood agar.
  • Leukotoxicity was evaluated using MTT dye reduction assays and Alamar blue tests with bovine neutrophils.

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Main Results:

  • Two of seven Pg isolates showed hemolysis on bovine blood agar; all seven were hemolytic on ovine blood agar.
  • All seven isolates demonstrated enhanced hemolysis on bovine blood agar via the CAMP reaction.
  • Preliminary assays indicated leukotoxicity in 13 of 16 Pg cultures (MTT assay), with all seven isolates showing leukotoxic activity (Alamar blue test).

Conclusions:

  • Pasteurella granulomatis isolates display variable hemolytic and leukotoxic properties.
  • The presence and degree of these hemolytic and leukotoxic activities may correlate with the pathogenicity of Pg in susceptible cattle.