The detection and characterization of genetically programmed responses to environmental stress

The rapid, accurate, and inexpensive detection of environmental contaminants (and the stress that they engender) is still a major problem worldwide. Though assays exist for monitoring these pollutants, they can often be expensive, time-consuming, and require extensive equipment and/or training in order to be effective. Research over the past decade has pointed to the measurement of enzymes encoded by genes programmed to respond to particular classes of environmental stress as a means of quantifying altered environmental health. The detection of physical and chemical contaminants can thus be performed using standard enzyme assays, by measuring the quantity of these enzymes (e.g. via immunoassays), or through the use of the technology of "gene-fusions." In this latter case, the genes encoding easily quantified enzymes are "fused" (cloned) such that their expression is under the control of genes whose expression is induced in the presence of these contaminants. In these cases, the measurement of the reporter gene's activity from the sample would signal the presence of a particular chemical and/or physical contaminant. The advantages of this system are its rapidity, ease of use, and single end-point measurement, thus allowing a "one box" (single detector) solution to measurements of environmental quality and health. Moreover, these systems can be designed for on-line monitoring and computer-aided operation for use in a wide variety of settings.