Related Experiment Video

Updated: Sep 22, 2026

Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays
08:39

Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays

Published on: January 3, 2015

Antisense oligonucleotide to insulin-like growth factor II induces apoptosis in human ovarian cancer AO cell line

D L Yin1, L Pu, G Pei

  • 1Shanghai Institute of Cell Biology, Chinese Academy of Sciences, China.

Cell Research
|July 21, 1998
PubMed

Insights

Antisense oligonucleotide targeting insulin-like growth factor II (IGFII) significantly reduced ovarian cancer cell proliferation and induced apoptosis. This suggests IGFII is a potential therapeutic target for ovarian cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Insulin-like growth factor II (IGFII) plays a role in cell proliferation and survival.
  • Aberrant IGFII signaling is implicated in various cancers, including ovarian cancer.
  • Targeting specific growth factors offers a potential strategy for cancer therapy.

Purpose of the Study:

  • To evaluate the antiproliferative and pro-apoptotic effects of antisense oligonucleotide to IGFII in human ovarian cancer cells.
  • To determine the potential of IGFII as a therapeutic target in ovarian cancer.

Main Methods:

  • Human ovarian cancer AO cells were treated with antisense oligonucleotide to IGFII.
  • Cell proliferation was assessed using 3H-thymidine incorporation.
  • Apoptosis was quantified by nuclear condensation and flow cytometry (propidium iodide staining).

Main Results:

  • IGFII antisense treatment (4.5 microM for 48 h) maximally inhibited AO cell proliferation.
  • Over 25% of cells treated with IGFII antisense (4.5 microM for 24 h) underwent apoptosis.
  • Control groups (IGFII sense or untreated) showed less than 3% apoptosis.

Conclusions:

  • Antisense oligonucleotide to IGFII effectively inhibits proliferation and induces apoptosis in human ovarian cancer cells.
  • IGFII represents a promising therapeutic target for ovarian cancer.
  • Antisense oligonucleotide to IGFII demonstrates potential as a novel therapeutic approach for ovarian cancer.

More Related Videos

Establishing Cell Lines Overexpressing DR3 to Assess the Apoptotic Response to Anti-mitotic Therapeutics12:28

Establishing Cell Lines Overexpressing DR3 to Assess the Apoptotic Response to Anti-mitotic Therapeutics

Published on: January 11, 2019

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer09:08

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Published on: January 12, 2020

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
Apoptosis01:30

Apoptosis

Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size reduction of the tissue.
The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...