Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Entamoeba dispar: ultrastructure, surface properties and cytopathic effect

M Espinosa-Cantellano¹, A Gonzáles-Robles, B Chávez

¹Departamento de Patología Experimental, Centro de Investigación y de Estudios Avanzados del IPN, Mexico. mespinosa@gwpat.pat.cinvestav.mx

The Journal of Eukaryotic Microbiology

|July 21, 1998

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Pair bonding and disruption impact lung transcriptome in monogamous Peromyscus californicus.

BMC genomics·2023

Same author

Mutations in the John Cunningham virus VP1 gene could predispose to the development of progressive multifocal leukoencephalopathy in multiple sclerosis patients undergoing treatment with natalizumab.

Multiple sclerosis and related disorders·2021

Same author

Molecular genetic analysis of AKR1C2-4 and HSD17B6 genes in subjects 46,XY with hypospadias.

Journal of pediatric urology·2020

Same author

Mutational analysis of SRD5A2: From gene to functional kinetics in individuals with steroid 5α-reductase 2 deficiency.

The Journal of steroid biochemistry and molecular biology·2020

Same author

Corrigendum to "isolation and sex steroid effects on the expression of the ATP-binding cassette transporter ABCB6 in Harderian glands of hamster (Mesocricetus auratus)" [Comparative Biochemistry and Physiology, Part A 232 (2019) 40-46].

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology·2019

Same author

Isolation and sex steroid effects on the expression of the ATP-binding cassette transporter ABCB6 in Harderian glands of hamster (Mesocricetus auratus).

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology·2019

Entamoeba dispar and Entamoeba histolytica exhibit distinct cytological features. E. dispar shows slower cytopathic effects on epithelial cells compared to E. histolytica.

Area of Science:

Microbiology
Cell Biology
Parasitology

Background:

Entamoeba dispar is a distinct species from Entamoeba histolytica, differentiated by biochemical and molecular criteria.
Understanding the cytological differences is crucial for accurate diagnosis and treatment.

Purpose of the Study:

To compare the cytological features of Entamoeba dispar and Entamoeba histolytica.
To elucidate the differences in their cytopathic effects on epithelial cells.

Main Methods:

Axenic culture of E. dispar and E. histolytica trophozoites.
Light and transmission electron microscopy for morphological analysis.
Toluidine blue staining for glycogen deposits.
Concanavalin A agglutination and cellular microelectrophoresis for surface properties.

Related Experiment Videos

Ussing chamber experiments to assess cytopathic effect on MDCK cell monolayers.

Main Results:

E. dispar trophozoites are more elongated with a single frontal pseudopodium and a noticeable uroid.
E. dispar exhibits cytoplasmic metachromasia due to glycogen deposits, unlike E. histolytica.
E. dispar has finer nuclear peripheral granules, a thinner surface coat, lower concanavalin A sensitivity, and higher negative surface charge.
E. dispar demonstrates a slower cytopathic effect on epithelial cells, with minimal phagocytosis compared to E. histolytica.

Conclusions:

Morphological and ultrastructural differences exist between E. dispar and E. histolytica.
E. dispar possesses a moderate but slower and less intense in vitro lytic capacity on epithelial cells compared to E. histolytica.