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Updated: Oct 13, 2026

Development of an In Vitro Assay to Evaluate Contractile Function of Mesenchymal Cells that Underwent Epithelial-Mesenchymal Transition
Published on: June 10, 2016
Transforming growth factor-beta-mediated proliferation of renal tubular epithelial cells involves pertussis
1Department of Renal Pharmacology, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406-0939, USA.
Abstract:
Exposure of LLCPK-1 cells to transforming growth factor (TGF)-beta(1) resulted in an increase in [3H]thymidine incorporation. This increase depended on the concentration of TGF-beta(1) used with an EC50 value of 0.3-1 pmol/l. Concentrations > 10 pmol/l were inhibitory. Similar effects occurred with TGF-beta(3). Addition of both TGF-beta(1) and TGF-beta(3) together had no additive effect. Pretreatment of cells with 10 mumol/1 genestein exerted no effect on TGF-beta-mediated response, whereas pretreatment with 10 mumol/1 Ro-32-0432 inhibited TGF-beta-mediated [3H]thymidine incorporation. In addition, pretreatment of cells with pertussis toxin attenuated TGF-beta-mediated proliferation of LLCPK-1 cells. These data indicate that in LLCPK-1 cells, TGF-beta(1) and TGF-beta(3) mediate their responses through the same receptor. This pathway involves protein kinase C and pertussis toxin-sensitive G protein.
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