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Antimicrobial susceptibilities of Campylobacter jejuni and coli by using E-test in Taiwan
Abstract:
To report the in vitro antibiotic susceptibility of Campylobacter species, we determined the MICs of 6 antibiotics by E-test for 93 human clinical strains and 35 chicken strains. The 6 antimicrobial agents tested were gentamicin, erythromycin, clindamycin, tetracycline, ciprofloxacin, and nalidixic acid. Isolates from humans were significantly more susceptible than chicken strains to erythromycin, clindamycin and ciprofloxacin. Nearly all of the human and chicken strains were susceptible to gentamicin. Among human isolates of C. jejuni, cross-resistance between nalidixic acid and ciprofloxacin was found in 66% of the strains, but none of the nalidixic acid-susceptible strains was resistant to ciprofloxacin. The higher prevalence of ciprofloxacin resistance in this area may be attributable to the large amount use of quinolones in poultry. Because of the high resistance rates of chicken isolates to the commonly used antimicrobial agents, it is necessary to create innovative methods to limit the inappropriate use of antibiotics in poultry in order to prevent the spread of the drug-resistant strains to humans.
Insights
Campylobacter antibiotic resistance is higher in chicken strains than human strains, particularly for ciprofloxacin. Limiting antibiotic use in poultry is crucial to prevent the spread of resistant bacteria to humans.
Area of Science:
- Microbiology
- Antimicrobial Resistance
- Food Safety
Background:
- Campylobacter species are significant human pathogens.
- Antibiotic resistance in Campylobacter poses a growing public health threat.
- Understanding resistance patterns in both human and animal isolates is critical.
Purpose of the Study:
- To determine the in vitro antibiotic susceptibility of clinical and poultry-derived Campylobacter strains.
- To compare resistance profiles between human and chicken isolates.
- To investigate the prevalence of specific antibiotic resistance, including quinolone resistance.
Main Methods:
- Minimum Inhibitory Concentrations (MICs) were determined using E-test.
- Tested 93 human clinical strains and 35 chicken strains.
- Evaluated susceptibility to gentamicin, erythromycin, clindamycin, tetracycline, ciprofloxacin, and nalidixic acid.
Main Results:
- Human isolates showed significantly higher susceptibility to erythromycin, clindamycin, and ciprofloxacin compared to chicken strains.
- Both human and chicken strains were largely susceptible to gentamicin.
- 66% of human C. jejuni isolates exhibited cross-resistance between nalidixic acid and ciprofloxacin.
Conclusions:
- High rates of antibiotic resistance in poultry Campylobacter strains are a concern.
- Widespread quinolone use in poultry may contribute to ciprofloxacin resistance.
- Innovative strategies are needed to reduce antibiotic misuse in poultry farming to mitigate the spread of resistant strains to humans.