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Related Experiment Videos

The covalent binding reaction of complement component C3

M Gadjeva1, A W Dodds, A Taniguchi-Sidle

  • 1The Medical Research Council Immunochemistry Unit, Department of Biochemistry, University of Oxford, United Kingdom.

Journal of Immunology (Baltimore, Md. : 1950)
|July 22, 1998
PubMed
Summary
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The complement system

Area of Science:

  • Immunology
  • Biochemistry

Background:

  • Complement component 3 (C3) covalent binding to pathogens is vital for complement-mediated immunity.
  • C3 activation involves acyl group transfer from a thioester to an acceptor molecule, forming an ester bond.

Purpose of the Study:

  • To investigate the reaction mechanism of C3 activation and covalent binding.
  • To determine if C3 utilizes a similar mechanism to human C4b.

Main Methods:

  • Biochemical assays to study C3 activation.
  • Analysis of intermediate bond formation during the reaction.

Main Results:

  • Evidence suggests C3 activation follows a two-step mechanism.
  • This mechanism involves an initial acyl-imidazole intermediate formation.

Related Experiment Videos

  • A histidine residue initiates the attack on the thioester, followed by cysteine-catalyzed acyl transfer.

    • Conclusions:

    • The two-step reaction mechanism observed in human C4b also occurs in C3.
    • This finding clarifies the molecular basis of C3's role in immune responses.