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Rapid fluorescence-based measurement of neutrophil migration in vitro
C W Frevert1, V A Wong, R B Goodman
1Medical Research Service of the Seattle VA Medical Center, Department of Medicine, University of Washington School of Medicine, 98108, USA. cfrevert@u.washington.edu
Abstract:
We have standardized a new chemotaxis chamber that uses fluorescence as the cellular marker for the measurement of leukocyte migration in vitro in disposable 96-well microplates. This new fluorescence-based assay is a robust assay because filter pore size, cell density, filter composition, and filter thickness do not affect PMN migration towards interleukin-8 or the complement fragment, C5a. When compared to two separate chemotaxis assays in which the migrated cells are counted visually, the fluorescence-based assay was more rapid, less labor intensive, and more sensitive. This new assay is a significant advance in the measurement of leukocyte migration in vitro.