Related Experiment Videos
Detection of human TNF-alpha mRNA by NASBA
B M Darke1, S K Jackson, S M Hanna
1Department of Medical Microbiology, University of Wales College of Medicine, Heath Park, Cardiff, UK.
Journal of Immunological Methods
|July 22, 1998
Abstract:
A method to amplify and detect TNF-alpha mRNA from primed Mono Mac 6 cells is described. A silica-based extraction system was utilised for preparation of cell extracts and specific oligonucleotide primers were designed for amplification of TNF-alpha mRNA by the NASBA process. Amplification products were detected using either a liquid hybridisation assay, with analysis by polyacrylamide gel electrophoresis, or a plate hybridisation system. The method has many potential applications for the study of inflammatory cytokines and cellular mRNAs in cell culture and clinical samples.