Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Peripheral nuclear matrix actin forms perinuclear shells

B H Clubb1, M Locke

  • 1Department of Zoology, The University of Western Ontario, London, Canada.

Journal of Cellular Biochemistry
|July 22, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Success and Survival of Composite Resin Restorations for the Management of Localized Anterior Tooth Wear: A Systematic Review and Meta-Analysis.

The European journal of prosthodontics and restorative dentistry·2024
Same author

Increased urokinase and consumption of α<sub>2</sub> -antiplasmin as an explanation for the loss of benefit of tranexamic acid after treatment delay.

Journal of thrombosis and haemostasis : JTH·2018
Same author

Teaching of implant dentistry in undergraduate dental schools in the UK and Ireland.

British dental journal·2018
Same author

An international collaborative study to calibrate the WHO 2nd International Standard for Ancrod (15/106) and the WHO Reference Reagent for Batroxobin (15/140): communication from the SSC of the ISTH.

Journal of thrombosis and haemostasis : JTH·2018
Same author

Salmonella Enteritidis with double deletion in phoP fliC and a competitive exclusion culture elicit substantial additive protective effects against Salmonella exposure in newly hatched chicks.

Vaccine·2017
Same author

Pyoderma gangrenosum with pathergy: A potentially significant complication following breast reconstruction.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2017
Same journal

Zinc Finger Proteins as Regulators of Organ Fibrosis.

Journal of cellular biochemistry·2026
Same journal

Intrinsic Disorder Status in Human Proteins Interacting With SARS-CoV-2 Proteins: Insights From Five Years of Translational Research.

Journal of cellular biochemistry·2026
Same journal

The Effect of Protein Tagging on Aggregation and Phase Separation.

Journal of cellular biochemistry·2026
Same journal

TRIM32 Alleviates the Inflammation in Spinal Cord Injury Progression Through Inducing the Ubiquitination Degradation of TLR4.

Journal of cellular biochemistry·2026
Same journal

Bedaquiline Binding at the Leading Site of Mycobacterium tuberculosis ATP Synthase Induces Distinct Structural and Dynamic Changes.

Journal of cellular biochemistry·2026
Same journal

Agrin Ablation in Osteoblasts Compromises Long Bone Structure and Osteoblastic Differentiation of Mesenchymal Stem Cells.

Journal of cellular biochemistry·2026
See all related articles

Perinuclear actin shells in 3T3 cells are located within the peripheral nuclear matrix. This finding clarifies the ultrastructural localization of these actin structures, previously unidentified at this level.

Area of Science:

  • Cell Biology
  • Cytoskeleton Dynamics
  • Nuclear Architecture

Background:

  • Perinuclear actin shells are observed across various organisms.
  • Previous studies identified these shells using phalloidin staining but lacked ultrastructural localization.
  • The precise subcellular compartment of these actin structures remained unknown.

Purpose of the Study:

  • To determine the ultrastructural localization of perinuclear actin shells in 3T3 cells.
  • To investigate the accessibility of nuclear actin to detection methods.

Main Methods:

  • Utilized 3T3 cell cultures.
  • Applied advanced microscopy techniques for ultrastructural analysis.
  • Investigated actin accessibility through various cellular extraction and digestion protocols.

Related Experiment Videos

Main Results:

  • Demonstrated that perinuclear actin shells reside within the peripheral nuclear matrix of 3T3 cells.
  • Showed that nuclear actin, including shells and matrix-associated actin, is generally inaccessible to antibodies and phalloidin.
  • Identified specific conditions (late mitosis, extracted interphase nuclei) for immunohistochemical detection of nuclear actin.

Conclusions:

  • The peripheral nuclear matrix is the specific location of perinuclear actin shells.
  • Standard immunohistochemical methods are insufficient for detecting nuclear actin due to its inaccessibility.
  • Modified protocols are required for effective nuclear actin visualization.