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Effect of Sulfo Lewis C on smoke inhalation injury in an ovine model
O Tasaki1, D W Mozingo, S Ishihara
1U.S. Army Institute of Surgical Research, San Antonio, TX 78234-6315, USA.
Objective:
To evaluate the effect of Sulfo Lewis C (SO3-3âGal1-3GlcNAc-O(CH2)8-COOMe), a putative ligand of selectins, on smoke inhalation injury.
Design:
Prospective animal study with concurrent controls.
Setting:
An animal laboratory.
Subjects:
Twelve 1-yr-old female sheep, weighing 24 to 33 kg.
Interventions:
Twelve sheep received nine exposure units of smoke generated by thermolysis of pine woodchips (80 g). Group 1 (n = 6) was untreated. Group 2 (n = 6) was treated with an intravenous infusion of Sulfo Lewis C after smoke exposure. Animals were killed 48 hrs after injury.
Measurements And Main Results:
Cardiopulmonary variables and blood gases were measured serially. Granulocyte free-radical production was measured before smoke exposure and at 4 and 48 hrs after injury. Ventilation/perfusion distribution (VA/Q) was analyzed using the multiple inert gas elimination technique. Granulocyte free-radical production was increased after smoke exposure in both groups. Oxygenation was significantly improved by the administration of Sulfo Lewis C. VA/Q analysis demonstrated significantly less blood flow to low VA/Q lung segments in treated animals.
Conclusions:
Selectin blockade attenuated lung injury after smoke exposure. These data support the hypothesis that neutrophils play a pivotal role in smoke inhalation injury.
