Related Experiment Video
Updated: Aug 13, 2026

Isolation of Microvascular Endothelial Tubes from Mouse Resistance Arteries
Published on: November 26, 2013
Actions of endothelin-1, 2, and 3 in the microvasculature of bone
P J Briggs1, C G Moran, M B Wood
1Mayo Graduate School of Medicine, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55905, USA.
Abstract:
Endothelins have recently been associated with hypoxia-related vascular smooth-muscle constriction and with the so-called no-reflow phenomenon following reperfusion. Their action is tissue dependent. The role of endothelins on vascular smooth muscle in bone is unknown. An ex vivo perfusion model was used to investigate the effects of the three different endothelins on the vascular resistance in the canine tibia. Endothelin-1 and endothelin-2 had molar potencies similar to that of norepinephrine, whereas endothelin-3 was less potent. Tachyphylaxis to the same dose of endothelin-1 did not occur. The calcium channel blocker, diltiazem, attenuated (45% reduction) the vasoconstrictor responses to norepinephrine (p < 0.005) but had a smaller effect (24% reduction) on the responses to endothelin (p < 0.025). Vascular smooth muscle in bone appears to have endothelin receptors that are similar to those in other organs.
Related Concept Videos
Paracrine Signaling
Mechanism of Angiogenesis
Regulation of Angiogenesis and Blood Supply
Intracellular Signaling Affects Focal Adhesions
Some...
Blood and Nerve Supply to the Bones
Nutrient Artery
The nutrient artery is the main blood vessel that enters the diaphysis via the nutrient foramen. While most long bones have only one nutrient foramen, large bones, such as the femur, may have two. This...
Acute Inflammation III: Local and Systemic Effects

