Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Infectious bursal disease virus polyprotein processing does not involve cellular proteases

F S Kibenge1, B Qian, J R Cleghorn

  • 1Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada.

Archives of Virology
|January 1, 1997
PubMed
Summary

Infectious Bursal Disease Virus (IBDV) polyprotein processing was studied. VPX is a primary product and does not mature into VP2, indicating cellular proteases are not involved in VPX to VP2 conversion.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multi-regional radiomics for predicting microvascular invasion and lymph node metastasis in intrahepatic cholangiocarcinoma.

Clinical radiology·2025
Same author

No evidence for metabolic adaptation during exercise-related energy compensation.

iScience·2024
Same author

Magnetic properties and critical behaviors of the nodal-line semimetal candidate ErIn<sub>3</sub>.

Journal of physics. Condensed matter : an Institute of Physics journal·2023
Same author

Anisotropic magnetoresistance and electronic features of the candidate topological compound praseodymium monobismuthide.

Physical chemistry chemical physics : PCCP·2023
Same author

[Association between inflammation activity of left atrial epicardial adipose tissue measured by <sup>18</sup>F-FDG PET/CT and atrial fibrillation].

Zhonghua xin xue guan bing za zhi·2021
Same author

Coupled electronic and magnetic relaxation in Fe<sub>1+</sub>Te: direct evidence for the interaction between itinerant carriers and local moments.

Journal of physics. Condensed matter : an Institute of Physics journal·2021

Area of Science:

  • Virology
  • Molecular Biology
  • Protein Processing

Background:

  • Infectious Bursal Disease Virus (IBDV) segment A encodes a polyprotein precursor.
  • The viral protease VP4 self-processes the polyprotein, but secondary products like VPX require further study.
  • The maturation pathway of VPX to VP2 remains undefined.

Purpose of the Study:

  • To investigate the processing and maturation of the IBDV polyprotein, specifically focusing on the fate of VPX.
  • To determine if VPX undergoes secondary processing to form VP2.
  • To elucidate the role of cellular proteases in VPX maturation.

Main Methods:

  • In vitro expression of IBDV cDNAs using rabbit reticulocyte lysates (coupled transcription-translation).
  • Expression using the Sindbis virus system in BHK-21 and Vero cell cultures.

Related Experiment Videos

  • Analysis of protein products via molecular mass identification and translational time course experiments.
  • Cycloheximide chase experiments to assess secondary processing.

    • Main Results:

    • Three main gene products, VPX (48 kDa), VP3 (34 kDa), and VP4 (30.5 kDa), were identified in both expression systems.
    • VPX, VP3, and VP4 are primary translation products, appearing within 20 minutes and peaking by 75 minutes.
    • No evidence of secondary processing of VPX to VP2 was observed in either expression system.
    • VP2 was not detected, suggesting VPX maturation to VP2 does not occur during foreign expression.

    Conclusions:

    • VPX, VP3, and VP4 are primary processing products of the IBDV polyprotein.
    • VPX does not appear to be a precursor to VP2.
    • Cellular proteases are not involved in the maturation of VPX to VP2.