Related Experiment Videos
Constitutive and inducible green fluorescent protein expression in Bartonella henselae
1Department of Microbiology and Immunology, Stanford University, Stanford, California 94305, USA. aklee@leland.stanford.edu
Infection and Immunity
|July 23, 1998
Abstract:
The green fluorescent protein (GFP) gene was expressed on a plasmid in B. henselae, and GFP-expressing bacteria were visualized by fluorescence microscopy. HEp-2 cells infected with GFP-expressing bacteria were separated from uninfected cells with a fluorescence activated cell sorter. Promoter fusions of B. henselae chromosomal DNA to gfp were examined by flow cytometry, and a B. henselae groEL promoter fusion which induced expression at 37 degreesC was isolated.