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Phospholipase C (EC 3.1.4.11): a malignancy linked signal transduction enzyme
1Laboratory for Experimental Oncology, Indiana University School of Medicine, Indianapolis 46202-5119, USA.
Anticancer Research
|July 23, 1998
Summary
Phospholipase C (PLC) activity increases with tumor growth in rats, indicating its role in cancer progression. Targeting PLC and related kinases may offer new cancer therapy strategies.
Area of Science:
- Biochemistry
- Oncology
- Cell Signaling
Background:
- Phospholipase C (PLC) is an enzyme involved in cell signaling.
- Its role in cancer development and progression is not fully understood.
Purpose of the Study:
- To investigate the relationship between PLC activity and tumor growth in rat hepatomas.
- To assess PLC activity in various normal rat organs.
Main Methods:
- Developed a standard isotopic method to measure PLC activity in plasma membrane fractions using phosphatidylinositol-4,5-bisphosphate (PIP2).
- Assayed PLC activity in transplantable rat hepatomas of varying growth rates and normal rat organs.
Main Results:
- PLC activity significantly increased with increasing growth rates in rat hepatomas.
- PLC activity was high in several normal organs but similar to liver in skeletal muscle.
- PLC activity showed nutritional regulation and a long half-life in bone marrow.
Conclusions:
- PLC is a transformation- and progression-linked signal transduction enzyme.
- Elevated PLC activity suggests enhanced signal transduction in rat hepatomas.
- Inhibiting PLC and related kinases could be a therapeutic strategy for cancer treatment.