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MAG and MOG enhance neurite outgrowth of embryonic mouse spinal cord neurons
1Development and Neurobiology Group, The Walter and Eliza Hall Institute of Medical Research, P.O. Royal Melbourne Hospital, Victoria, Australia.
Abstract:
Myelin-associated glycoprotein (MAG) inhibits neurite outgrowth of postnatal spinal cord neurons, but its effect on embryonic neurons is unknown. The effect on neurite outgrowth of another myelin protein, myelin-oligodendrocyte glycoprotein (MOG) is also unknown. We determined the effect of MAG and MOG on embryonic day 17 spinal cord neurons, which were cultured on MAG, MOG or control transfected CHO cells. Neurite outgrowth was examined and both total neurite length and longest neurite length were significantly enhanced by both MAG and MOG. These findings show that, in contrast to postnatal spinal cord neurons, MAG can enhance neurite outgrowth of embryonic spinal cord neurons. In addition, another myelin protein, MOG, can also modulate neurite outgrowth.
Insights
Myelin-associated glycoprotein (MAG) and myelin-oligodendrocyte glycoprotein (MOG) promote embryonic spinal cord neuron growth, unlike MAG
Area of Science:
- Neuroscience
- Developmental Biology
- Cell Biology
Background:
- Myelin-associated glycoprotein (MAG) is known to inhibit neurite outgrowth in postnatal spinal cord neurons.
- The effects of MAG and myelin-oligodendrocyte glycoprotein (MOG) on embryonic spinal cord neurons are not well understood.
Purpose of the Study:
- To investigate the influence of MAG and MOG on neurite outgrowth in embryonic spinal cord neurons.
- To compare the effects of MAG on embryonic versus postnatal neurons.
Main Methods:
- Culturing embryonic day 17 spinal cord neurons.
- Exposing neurons to MAG, MOG, or control Chinese hamster ovary (CHO) cells.
- Quantifying neurite outgrowth, including total and longest neurite length.
Main Results:
- Both MAG and MOG significantly enhanced total neurite length and longest neurite length.
- MAG promoted neurite outgrowth in embryonic neurons, contrasting with its inhibitory effect on postnatal neurons.
Conclusions:
- MAG enhances neurite outgrowth in embryonic spinal cord neurons.
- MOG also modulates neurite outgrowth in embryonic spinal cord neurons.
- These findings suggest developmental stage-dependent roles for myelin proteins in neural development.