Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA breakage and repair

P A Jeggo1

  • 1MRC Cell Mutation Unit, University of Sussex, Brighton, United Kingdom.

Advances in Genetics
|July 25, 1998
PubMed
Summary
This summary is machine-generated.

Mammalian cells repair DNA double-strand breaks (DSBs) using nonhomologous end joining (NHEJ). Key proteins like DNA-PK, DNA ligase IV, and XRCC4 are involved, with potential roles for yeast Sir proteins in higher organisms.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DNA double-strand break repair in a cellular context.

Clinical oncology (Royal College of Radiologists (Great Britain))·2014
Same author

DNA DSB repair pathway choice: an orchestrated handover mechanism.

The British journal of radiology·2013
Same author

Endogenously induced DNA double strand breaks arise in heterochromatic DNA regions and require ataxia telangiectasia mutated and Artemis for their repair.

Nucleic acids research·2011
Same author

CsA can induce DNA double-strand breaks: implications for BMT regimens particularly for individuals with defective DNA repair.

Bone marrow transplantation·2008
Same author

DNA double-strand breaks: their cellular and clinical impact?

Oncogene·2007
Same author

Accelerated telomere shortening and telomere abnormalities in radiosensitive cell lines.

Radiation research·2005

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Mammalian cells primarily utilize nonhomologous end joining (NHEJ) for DNA double-strand break (DSB) repair, distinct from yeast mechanisms.
  • Recent identification of key proteins such as DNA-PK, DNA ligase IV, and XRCC4 offers insights into the NHEJ pathway.

Purpose of the Study:

  • To elucidate the molecular mechanisms of NHEJ in mammalian cells.
  • To investigate the potential role of yeast Sir proteins in higher organisms' DNA repair pathways.
  • To explore the interactions between DNA repair and damage response mechanisms.

Main Methods:

  • Identification and characterization of proteins involved in NHEJ.
  • Comparative studies between yeast and mammalian DNA repair pathways.

Related Experiment Videos

  • Analysis of overlapping defects in telomere maintenance in yeast and human cell lines.

    • Main Results:

    • Several proteins, including DNA-PK, DNA ligase IV, and XRCC4, are crucial components of the mammalian NHEJ pathway.
    • Yeast Sir proteins (Sir2, -3, -4) are implicated in DNA repair and may play a role in higher organisms.
    • Evidence suggests functional overlap between DNA repair and damage response pathways, as seen in telomere maintenance defects.

    Conclusions:

    • NHEJ is a critical, potentially error-free, DNA repair mechanism in mammals, involving a complex interplay of proteins.
    • Further research is needed to fully understand the accuracy and efficiency of NHEJ, especially for complex DSBs.
    • Investigating the interactions between DNA repair pathways and damage response mechanisms is a key future challenge.