Related Experiment Video
Updated: Aug 15, 2026

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
Published on: March 24, 2015
Heterocomplexes of human interferon and immunoglobulins: formation and properties
Abstract:
To form heterocomplexes, human diploid fibroblast interferon was reacted with an excess of human immunoglobulin in the presence of an optimal concentration of glutaraldehyde. There was virtually no loss of interferon activity due to this treatment and the resulting heterocomplexes possessed the biological properties of both native interferon and immunoglobulin molecules. The apparent molecular weights determined by gel electrophoresis in the presence of sodium dodecyl sulfate were 20,000 for native interferon and 0.5 to 1 X 10(6) for the heterocomplex. Native interferon injected into mice intramuscularly had a serum half life of about 5 hours. However, serum interferon was not detected in mice receiving a similar dose of the heterocomplex.
More Related Videos
Related Concept Videos
Antibody Structure
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Protein Complex Assembly
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Protein Complexes with Interchangeable Parts
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Complexes with Interchangeable Parts
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Antibody Structure and Classes
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
Inhibitors of Viral Protein Synthesis

