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Related Experiment Videos

Osmosensitive C1- currents and their relevance to regulatory volume decrease in human intestinal T84 cells: outwardly

T D Bond1, S Ambikapathy, S Mohammad

  • 1Physiology Group, Biomedical Sciences Division, King's College London, Strand, London WC2R 2LS, UK.

The Journal of Physiology
|July 29, 1998
PubMed
Summary
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The swelling-activated chloride current (ICl(swell)) is crucial for regulatory volume decrease (RVD) in T84 cells, as it is sensitive to tamoxifen, DDFSK, and DIDS, but not Cd2+. This contrasts with the hyperpolarization-activated current, which is Cd2+-sensitive.

Area of Science:

  • Cellular physiology
  • Ion transport mechanisms
  • Human intestinal cell function

Background:

  • T84 human intestinal cells exhibit regulatory volume decrease (RVD) in response to osmotic stress.
  • Understanding the specific ion channels involved in RVD is critical for comprehending cellular volume regulation.

Purpose of the Study:

  • To elucidate the specific chloride currents responsible for RVD in T84 cells.
  • To differentiate between swelling-activated and hyperpolarization-activated chloride currents and their roles in RVD.

Main Methods:

  • Electrophysiological recordings of chloride currents (ICl(swell)) in T84 cells.
  • Pharmacological inhibition using tamoxifen, cadmium chloride (Cd2+), 1,9-dideoxyforskolin (DDFSK), and 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid (DIDS).

Related Experiment Videos

  • Osmotic challenge experiments to induce hyposmotic shocks and assess RVD.

    • Main Results:

    • Swelling-activated outwardly rectifying chloride current (ICl(swell)) was blocked by tamoxifen, DDFSK, and DIDS, but not Cd2+.
    • A hyperpolarization-activated, Cd2+-sensitive chloride current, resembling ClC-2, was identified but not significantly involved in RVD.
    • T84 cells demonstrated robust RVD following hyposmotic shocks, which was inhibited by tamoxifen, DDFSK, and DIDS.

    Conclusions:

    • The primary anionic pathway for RVD in T84 cells is the swelling-activated ICl(swell).
    • This ICl(swell) is sensitive to tamoxifen, DDFSK, and DIDS, highlighting its distinct nature from the hyperpolarization-activated, Cd2+-sensitive current.