Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pi-class glutathione S-transferase: regulation and function

C J Henderson1, A W McLaren, G J Moffat

  • 1Ninewells Hospital and Medical School, ICRF Molecular Pharmacology Unit, Dundee, UK. m.rooney@icrf.icnet.uk

Chemico-Biological Interactions
|July 29, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction: The potent human CAR activator CITCO is a non-genotoxic hepatic tumour-promoting agent in humanised constitutive androstane receptor mice but not in wild-type animals.

Archives of toxicology·2026
Same author

The potent human CAR activator CITCO is a non-genotoxic hepatic tumour-promoting agent in humanised constitutive androstane receptor mice but not in wild-type animals.

Archives of toxicology·2025
Same author

Defining the Contribution of CYP1A1 and CYP1A2 to Drug Metabolism Using Humanized CYP1A1/1A2 and Cyp1a1/Cyp1a2 Knockout Mice.

Drug metabolism and disposition: the biological fate of chemicals·2019
Same author

An Extensively Humanized Mouse Model to Predict Pathways of Drug Disposition and Drug/Drug Interactions, and to Facilitate Design of Clinical Trials.

Drug metabolism and disposition: the biological fate of chemicals·2019
Same author

Quantifying the influence of small omnivorous fishes on seagrass epiphyte load.

Journal of fish biology·2016
Same author

Assessing the suitability of a non-lethal biopsy punch for sampling fish muscle tissue.

Fish physiology and biochemistry·2016

Glutathione S-transferase pi (GST pi) is linked to cancer and drug resistance. Researchers created a mouse model lacking GST pi to study its functions and impact on xenobiotic metabolism.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oncology

Background:

  • Glutathione S-transferase pi (GST pi) over-expression is linked to various human cancers and poor prognosis.
  • GST pi plays a role in antineoplastic drug resistance.
  • GST pi gene expression is regulated, with polymorphisms associated with cancer risk.

Purpose of the Study:

  • To investigate the endogenous functions of GST pi.
  • To understand the role of GST pi in carcinogenesis.
  • To create a mouse model for studying GST pi deficiency.

Main Methods:

  • Utilized a multi-drug resistant human mammary carcinoma cell line (MCF7 VCREMS) to study GST P1 transcriptional regulation.
  • Investigated GSTP1 locus polymorphisms (GSTP1a and GSTP1b) and their association with cancer.

Related Experiment Videos

  • Generated a mouse strain deficient in GST pi expression using homologous recombination in embryonic stem cells.

    • Main Results:

    • GST pi over-expression is associated with carcinogenesis and drug resistance.
    • GSTP1b allele linked to increased bladder and testicular cancer; GSTP1a allele decreased in prostate cancer.
    • Created a mouse model completely lacking GST pi expression.

    Conclusions:

    • GST pi is implicated in cancer development, prognosis, and drug resistance.
    • GST pi polymorphisms may influence cancer susceptibility.
    • The developed mouse model offers a novel tool to study the endogenous functions of GST pi and its effects on xenobiotic metabolism.