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Stem cell factor influences mast cell mediator release in response to eosinophil-derived granule major basic protein
G T Furuta1, S J Ackerman, L Lu
1The Combined Program in Pediatric Gastroenterology and Nutrition, The Children's Hospital and Massachusetts General Hospital, Boston, MA, USA.
Blood
|July 29, 1998
Summary
Stem cell factor (SCF) promotes mast cell maturation. Mature mast cells stimulated with eosinophil-derived major basic protein (MBP) release inflammatory mediators, unlike less mature cells.
Area of Science:
- Immunology
- Cell Biology
Background:
- Stem cell factor (SCF) is crucial for mast cell development and survival.
- Mast cells play a key role in allergic and inflammatory responses.
Purpose of the Study:
- To investigate the effect of SCF on mast cell differentiation and responsiveness to eosinophil-derived major basic protein (MBP).
Main Methods:
- Differentiated mouse bone marrow-derived mast cells (BMCMC) using SCF or conditioned medium (CM).
- Compared mature BMCMC in SCF with immature BMCMC in CM and a mast cell line (Cl.MC/C57.1).
- Stimulated mast cells with MBP and a poly-L-arginine analog, measuring mediator release.
Main Results:
- SCF-treated BMCMC showed characteristics of mature mast cells (staining, protease, histamine, serotonin uptake).
- SCF-treated BMCMC released serotonin, arachidonic acid metabolites, and TNF-alpha upon MBP stimulation.
- Immature mast cells and the cell line did not respond to MBP but did respond to poly-L-arginine.
Conclusions:
- SCF significantly enhances mast cell differentiation.
- SCF-primed mast cells exhibit increased responsiveness to eosinophil-derived MBP, indicating a role in allergic inflammation.