Determination of fluoride in bovine urine

Fluoride concentration in bovine urine is determined by using a selective ion electrode. Urine is buffered and measured against known fluoride (F) standards. The method is applicable to 2 to 40 mg F/L without further dilution. Typical normal urine contains less than 10 mg F/L. Concentrations greater than 10 mg/L support a clinical diagnosis of fluorosis in cattle. Repeatability coefficient of variation (CV) ranged from 1.6 to 3.5% for F concentrations of 3.2-43 mg/L. Reproducibility CV ranged from 0.3 to 7.3% for F concentrations of 7.0-17.2 mg/L.