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Effects of transforming growth factor alpha and hepatocyte growth factor on acid-induced damage in rat gastric
O Furukawa1, H Matsui, N Suzuki
1Department of Applied Pharmacology, Kyoto Pharmaceutical University, Yamashina, Japan.
Abstract:
We examined the effects of transforming growth factor alpha (TGF-alpha) and hepatocyte growth factor (HGF) on acid-induced damage in a rat gastric epithelial cell line. Pretreatment of cells for 30 min with TGF-alpha, but not HGF, significantly prevented the acid-induced cell damage in a concentration-dependent manner. Genistein significantly reduced the protective effect of TGF-alpha. DNA synthesis in RGM1 cells was increased when the cells were incubated with TGF-alpha and HGF for 24 hr. We conclude that the protective effect of TGF-alpha against acid-induced damage seems to be caused by the activation of Na+/H+ exchangers and not by enhanced DNA synthesis.